Journal: AoB Plants

Article Title: Reconstructing a herbivore’s diet using a novel rbc L DNA mini-barcode for plants

doi: 10.1093/aobpla/plx015

Figure Lengend Snippet: Flow chart of procedures for collecting, extracting and quantifying DNA used to identify deer forage species and deer identification for 12 samples. (1) Samples were collected within a 1 km 2 at Smithsonian’s Conservation Biology in Front Royal, VA in October 2014. (2) The DNA extraction was conducted using Zymo Fecal DNA Mini-prep Kit. (3a) Known microsatellite markers were used verify each of the 12 samples were from separate individuals. (3b) In parallel to the PCR based mini-barcode approach, WGS data was generated and analysed using five whole chloroplast sequences in CLC Bio. (3c) PCR was conducted using primers designed for mini-Barcode rbc l region. (4a) Sequencing of amplicons was performed on an Illumina MiSeq V2. (4b) Voucher DNA Barcode samples from plant species collected in region were obtained from GenBank. (5) Data quality control was performed in CLC Bio Genomics Workbench (ver. 7.4) while bioinformatics analysis, where sequences were assigned taxonomic identity, were performed using Qiime. (6) Primary statistics contrasting animal diet included PERMANOVA and ANOSIM which utilized OTU tables from Qiime analysis.

Article Snippet: The Next Generation Sequencing library for the 36 fecal pellet PCR samples followed the Illumina 16S protocol (see ).

Techniques: DNA Extraction, Generated, Sequencing

Journal: Microbiology Spectrum

Article Title: Host Bias in Diet-Source Microbiome Transmission in Wild Cohabitating Herbivores: New Knowledge for the Evolution of Herbivory and Plant Defense

doi: 10.1128/Spectrum.00756-21

Figure Lengend Snippet: The putative phenotype in the microbiome in this study. (A) Proportion of putative aerobic. (B) Proportion of putative anaerobic. The Mann-Whitney-Wilcoxon test was used to assess the hypothesis of whether the proportion of aerobic microbiome in either insect gut microbiome or diet-source microbiome was significantly higher than that in the milu gut microbiome. Herbivorous insects included leaf-eating grasshoppers and locusts in the Dafeng region. Diet included dietary plants for the insects and milu in the Dafeng region.

Article Snippet: We collected the sympatric insects (grasshoppers and locusts) and milu fecal samples in the wetlands of Dafeng (Jiangsu Province) ( ; see also Table S1 in the supplemental material).

Techniques: MANN-WHITNEY